This option has nothing to do with equalizing the difference in levels between the 2 channels, so you needn't worry.
Audio waveforms are "Alternating Current" & should be, on average, symmetrical about zero. Any "Direct Current" offset will show up as a vertical shift in the position of the waveform for that channel. If this is very obvious, it can lead to premature clipping of the signal as, being shifted, it will hit one end-stop before the other.
Leaving the "remove offset" box checked certainly won't do any harm, as it's good policy to remove any offset before normalizing/exporting the final file, if only to prevent clicks & pops when starting & stopping playback.
If you do see a significant offset, which is quite rare, it's usually caused by some anomaly in the mic-pre stage, often due to mic power leaking into the input through a faulty capacitor or suchlike.