when I use CD Wave to cut up 24 bit files, I always check the "use alternate 24-bit format"
It's something about Microsoft trying to redefine the way header info is used in .wav files (I forget exactly what they were trying to change and why). but in my experience, a file saved from CD Wave, WITHOUT using the "alternate" 24-bit format, will NOT open in Cool Edit 2000. However, when I use the "alternate" 24-bit format, Cool Edit opens it just fine, and I've had no problems compressed to FLAC.